Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 1 Histogram of adsorbed (A) NT4 and (B) PAMAM nanoparticles (PBS, 0.15 M, pH 7.4) indicated by direct AFM enumeration obtained for low surface-molecule concentration. Notes: Figure created by taking into account ten randomly chosen areas, where each micrograph of the NT4 or PAMAM monolayer at the mica surface has a size of 0.5×05 µm. Insets show the NT4 monolayer (A) and PAMAM monolayer (B) on mica, for surface concentration of individual molecules of approximately 800/µm2. Abbreviations: PAMAM, polyamidoamine; AFM, atomic force microscopy.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Concentration Assay, Microscopy

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 3 Dependence of ζ-potential of PAMAM nanoparticles on bulk concentra- tion of NT4 in the suspension after mixing cb. Notes: Points show experimental results obtained by laser Doppler velocimetry for 0.15 M PBS (pH 7.4) and PAMAM concentration 50 mg•L−1. Solid lines show linear fit of data. Dashed lines show ζ-potential of bare microparticles. Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Suspension, Concentration Assay

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 2 Adsorbed NT4–PAMAM (PBS, 0.15 M, pH 7.4) indicated by direct AFM enumeration obtained for low surface-molecule concentration. Note: Figure created by taking into account ten randomly chosen areas, where each micrograph of the NT4–PAMAM monolayer at the mica surface has a size of 1×1 µm. Abbreviations: PAMAM, polyamidoamine; AFM, atomic force microscopy.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Concentration Assay, Microscopy

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 4 Dependence of residual NT4 concentration after adsorption step c* at PAMAM nanoparticles on initial protein concentration. Notes: Determined by ELISA at pH 7.4 in 0.15 M PBS after 24 hours' adsorption. Bars show data for bounded NT4 (gray) and residual NT4 (green). Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Concentration Assay, Adsorption, Protein Concentration, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 6 Desorption characteristics of NT4 proteins in vitreous body after injection of control mice (before treatment), NaIO3 injection, and NaIO3–PAMAM or NaIO3– PAMAM–NT4 injection. Notes: (A) ELISA detection of NT4; (B) NT4 concentration normalized to total protein concentration of individual tissue samples. Results shown as means ± SD. *P<0.05; **P<0.01; ***P<0.001. Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Injection, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Protein Concentration

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 5 Desorption characteristics of NT4 from PAMAM G6 dendrimers for PBS electrolytes. Notes: NT4 detection by ELISA over the first (A) 10 hours and (B) 32 days of incubation. Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 7 Desorption characteristics of NT4 proteins in retina after injection of control mice (before any treatment), NaIO3 injection, and NaIO3–PAMAM or NaIO3– PAMAM–NT4. Notes: (A) ELISA detection of NT4; (B) NT4 concentration normalized to total protein concentration of individual tissue samples. Results shown as means ± SD. *P<0.05; **P<0.01; ***P<0.001. Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Injection, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Protein Concentration

Journal: International Journal of Nanomedicine

Article Title:

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo

doi: 10.2147/ijn.s210140

Figure Lengend Snippet: Figure 8 Confocal images showing NaIO3-injured mouse eyes (vitreous body and retina) after administration of fluorescently-labeled NT4–PAMAM conjugates. Notes: Top middle image reveals the presence of green fluorescent particles that may correspondto nanocomplexes in the vitreous body only 7 days after NT4–PAMAM injection. Particles were undetectable in the retina at all study time points (bottom row). Magnification 63×, oil immersion. Blue fluorescence, nuclei (DAPI); green background, tissue autofluorescence. Abbreviation: PAMAM, polyamidoamine.

Article Snippet: Filtered (Centrifree ultrafiltration device; Merck, Darmstadt, Germany) stock solutions of carrier-free recombinant human NT4 (268-N4-025/CF; R&D Systems) of known concentrations (typically 250 mg•L−1) in PBS (pH 7.4 ±0.2; Biomed, Lublin, Poland) were prepared to remove aggregates and provide a constant, free-form protein-molecule concentration in the solvent.

Techniques: Labeling, Injection